ATOMIC ABSORPTION SPECTROMETER ( AAS )

    What is AAS :-

• AAS abbreviation is atomic absorption spectrometer.

• AAS is a machine used to detect the element such as metal and minerals.

• It measures different types of matrix (food, pharmaceutical, water chemical and etc.)

Principle of AAS :-

• AAS works based on Beer-Lambert's law.

• It says that absorption is directly proportional the path length and concentration of the solution.

• In AAS we can analyze elements in both the way absorption as well as emission.

AAS Instrumentation:-

• Air compressor  (for air)

• Acetylene (fuel gas)

• Nitrous oxide (high temperature)

• HVG (for lower concentration( ppb )

• Exhaust

• Nebulizer 
for the conversion of liquid to aerosol

• Bruner
 To ignite flame (normal flame 10 cm high flame 05 cm) aerosol converts to atomization.

• Lamps
 To emit a beam of light  for respective element atoms absorb energy and electrons of atom jumps from ground state to exited state

• Monochromator
To separate particular beam

• Detector
detect the amount of absorbed light and gives its concentration.

AAS diagram representation :-

                                           Exhaust
                                      ↑
    Detector   ←      Brunel      ←   Monochromator    ←    lamps
                               
                                      ↑

                                  nebulizer
                     
                                      ↑

                          aspirate solution

AAS linearity calibration graph :-

Linearity plotted on Y-axis absorbance V/S X-axis concentration of the solution

AAS application :-

• AAS is used to detect metals in various matrix.

• AAS used to detect minerals.

• By using HVG we can detect lower limit for toxic metals at ppb level.

• AAS used to detect toxic metals.