CHEMISTRYWITHFUN

What is HPLC :-     1. Originally referred to as High-Pressure Liquid Chromatography.     2. Now more commonly called  High  Performance Liquid Chromatography.     3. HPLC is really the automation of traditional liquid chromatography under conditions which provide for enhanced separations during shorter periods of time, utilizing very small particles , small column diameters, and very high fluid pressure. Principle of HPLC :-        Separation of Compounds takes place based on polarity under high pressure of mobile-phase and column through which compound eject with respect to time (RT). Stationary of HPLC (column) :- Polar (“Normal”  Phase) ex :  Silica, alumina Non-Polar (“Reversed Phase”) ex:  ODS Silica gel,  – C18, C8   Mobile Phase of HPLC (Solvent) :- 1. Normal chromatography    Hexane ; dichloromethane; iso...

    What is AAS :- •  AAS abbreviation is atomic absorption spectrometer. •  AAS is a machine used to detect the element such as metal and minerals. •  It measures different types of matrix (food, pharmaceutical, water chemical and etc.) Principle of AAS :- •  AAS works based on Beer- Lambert's  law. •  It says that absorption is directly proportional the path length and concentration of the solution. •  In AAS we can analyze elements in both the way absorption as well as emission. AAS Instrumentation:- •  Air compressor  (for air) •  Acetylene (fuel gas) •  Nitrous oxide (high temperature) •  HVG (for lower concentration( ppb ) •  Exhaust •  Nebulizer  for the conversion of liquid to aerosol •  Bruner  To ignite flame (normal flame 10 cm high flame 05 cm) aerosol converts to atomization. •  Lamps  To emit a ...